Coherent diffraction imaging: application to test and biological samples
by
Enju Lima, ESRF Grenoble
→
Europe/Berlin
Bldg. 25b, 109
Bldg. 25b, 109
Description
Coherent Diffraction Imaging (CDI) provides a method of imaging non-crystalline samples from the coherent diffraction data by recovering the lost phase in the diffraction intensity through an iterative algorithm. It employs no lens that would limit the resolution and the depth of focus, nor is it restrictive to a specific sample type. Thus a broad range of samples can be imaged, whether crystalline or non-crystalline, thick or thin. These advantages allow CDI to have the potential to achieve high-resolution imaging down to the diffraction limit in biological samples. However, the challenges are (1) data collection with a high signal-to-noise ratio, especially with weak scattering samples (2) sample preparation for biological samples. This talk will present (1) the recent experiment with tungsten test samples at ID 10 A, ESRF using 7.3 keV x-rays and, (2) the application of CDI to biological samples, freeze-dried yeast cells and frozen-hydrated yeast spores using soft x-rays conducted at ALS, Berkeley.
