Combining nanobeam techniques to study transport processes in biomedical tissues
by
Walter Schröder, Institute Phytosphere, Forschungszentrum Jülich
→
Europe/Berlin
Bldg. 25b, room 109
Bldg. 25b, room 109
Description
Modern techniques for the mapping of elements and isotopes in biomedical tissues are typically highly specialized. Focused beams of photons, electrons or ions interact with analytes differently in specific ways. The differing physical interactions give rise to obtain certain types of information, resulting in specific advantages like high detection sensitivity, extensive dynamic range, high spatial resolution or the ability to differentiate isotopes by high mass resolution. The advantages come along with inherent disadvantages like strong matrix effects or lack of quantification. Therefore the aim is to combine techniques of different physical principles to analyze the same sample to complement the necessary information.
A complete cryogenic workflow was developed to integrate sample preparation by shock freezing with low temperature analysis using SIMS, SEM and EDXA. These techniques are supplemented with synchrotron based X-ray tomography and x-ray – fluorescence. The application examples provide a new view of the transport routes of water and inorganic solutes in the long range transport in higher plants.
