CMWS Seminar | How Hydrophilic are Hydrophobic Hydrogels from Disordered Proteins? From Phenomenological to (Ultrafast) Mechanistical Studies

by Prof. Simone Techert (University of Göttingen and DESY)




By definition, a hydrogel is “a crosslinked hydrophilic polymer that does not dissolve in water. They are highly absorbent yet maintain well defined structures.” (Encyclop. Chem. Technol.). Since proteins are also polymers, in this lecture I will present optical and X-ray studies of an almost 100% intrinsically disordered protein that belongs to the group of nuclear pore proteins. According to the properties of this disordered protein, it cannot adopt protein-typical well-defined second- ary structure elements, which are also required for hydrogel formation. Nevertheless, this very hydrophobic protein immediately forms hydrogels when dissolved in water. Time-resolved optical and X-ray studies will lead us out of this paradox. I will discuss how this protein, due to its very specific interaction with water, forms metastable and intermediate structures that define its intermediates and metastability in its coating function. The found autocatalytic mechanism of self-assembly will be embedded in a Schuster-Eigen hypercycle including the femto/picosecond to minutes time regime. Finally, I will discuss the influence of Hofmeister's series on the structure of water - and whether and how "salting-in" and "salting-out" affects hydrophilicity - and thus hydrogel formation.

CMWS webpage: Abstract and access link (see pdf-file)


Overview of presented X-ray Methods:

[1] Ultrafast Time Structure Imprints in Complex Chemical and Biochemical Reactions, Chapter 15 in U. Bergmann et al. (eds.), Fundamentals and Application of Free Electron Lasers, Royal Chemical Society, Oxford, 301-322, (2017).

[2] Development of Ultrafast X-ray Free Electron Laser Tools in (Bio)Chemical Research, in T. Salditt et al. (eds.), Nanoscale Photonic Imaging, Springer Series: Topics in Applied Physics 134 (2020).

[3] Structure Time Correlations Studied with Pulsed High-flux X-ray Sources, in K. Amini et al. (eds.) Structural Dy- namics with X-Ray and Electron Scattering, AIP-Publishing (2021 / 2022).