The good, the bad, the weakly -- studying bio-pharmaceutical relevant complexes with small angle X-ray scattering at P12

by Melissa Graewert (EMBL Hamburg)

Tuesday 22 Nov 2022, 13:15 → 14:00 Europe/Berlin

Online

Small angle X-ray scattering (SAXS) is a universal and powerful method to analyze proteins and other macromolecules in solution over a broad range of conditions. It has become highly attractive for the pharmaceutical industry as a high-throughput method. SAXS provides direct insights into the quaternary state and structural responses to the effects of buffer composition, to interactions with other macromolecules or small ligands. 
Here, I present current and future structural investigations in terms of complex formation. Emphasis is put on adapting our experimental setups at P12 to address different challenges posed by the various properties of macromolecular complexes. 

This line of work is put into context of the current fast-paced developments in the field of structural biology (ie. cryo-EM and AI-based modeling) as well as the benefit of close collaboration with industrial partners.