Exploring how proteins move within cells is essential for understanding various biological processes, such as reaction rates and transport of molecules within the cytoplasm. Here I will present results from our recent study, which addresses the influence of hydrodynamic interactions on the diffusion of proteins on the molecular scale. By employing advanced protein megahertz X-ray Photon...
The new 4th generation storage ring sources, such as the MAX IV Laboratory 3 GeV ring, gives new possibilities to study dynamics using crystallography. The MicroMAX beamline that recently started its user operation is designed to be flexible both in terms of X-ray beam and experiment setup. The focus is on serial and time-resolved crystallography but with state-of-the-art functionality also...
Photolyase is an enzyme that uses light to catalyze DNA repair. To capture the reaction intermediates involved in the enzyme’s catalytic cycle, we conducted a time-resolved crystallography experiment. We found that photolyase traps the excited state of the active cofactor, flavin adenine dinucleotide (FAD), in a highly bent geometry. This excited state performs electron transfer to damaged...
Serial femtosecond crystallography (SFX) is a method for obtaining damage-free diffraction patterns of protein microcrystals even under near-physiological temperature conditions by utilizing intense, ultrafast X-ray free-electron laser (XFEL) pulses$^{1,2}$. Moreover, SFX is applicable to analyze chemical reactions initiated by various triggers, such as substrates, ligands, heat, and light, on...
Structure determines function, and one of the great dream experiments in science is to directly observe atomic motions during the defining moment when matter transforms from one state to another and witness the key reaction modes directing chemistry. X-ray diffraction (XRD) on solid-state samples provides direct information on how atoms specifically respond and rearrange to stabilize the...
The macromolecular crystallography (MX) beamline I04 [1] at Diamond has evolved over time through various upgrade projects that aimed at increasing scientific capability but at the same time aiming for increased stability so that the best possible data can be obtained by the user. We have deviated significantly from the initial concept for beam delivery and are now providing beam through the...
