The Three-way Meeting brings together scientists and engineers from the high-energy synchrotron radiation sources, APS, ESRF, PETRA III, and SPring-8. The title of the meeting reflects the three continents, America, Asia and Europe, hosting high-energy third generation sources. The meeting takes place roughly every 18 months. It is a chance for staff from the four X-ray facilities to discuss, compare, and coordinate research and development between the four synchrotron radiation sources. This 16th Three-way Meeting and its associated Optics Workshop will take place at DESY from September 14 to 16, 2016. The Optics Workshop will be held on the first day, followed by the Three-way Meeting on the following two days.