Synchrotron Radiation for Bio-Imaging at PETRA III

DESY, Notkestraße 85, 22607 Hamburg, Bldg. 28c, seminar room

DESY, Notkestraße 85, 22607 Hamburg, Bldg. 28c, seminar room


Single isolated neural receptor cell in X-ray light: Distribution of K (red) and Cl (blue) [Dučić et al, 2009].

This meeting aims to bring scientists from biological and medical backgrounds together with synchrotron experts to establish a new interdisciplinary user community around the new high resolution beamlines P11 and P06 at PETRA III. Both beamlines are currently under construction and will enter the commissioning phase shortly after the workshop. The workshop is a unique opportunity for a hands-on introduction into the new possibilities for bio-imaging on the micro- and nano-scale at this facility. It will also be an excellent occasion to propose new experiments and collaborations, as well as to discuss future developments or requirements from the users. We are looking forward to addressing novel, groundbreaking biological and medical questions using synchrotron radiation.

We propose to offer opportunities such as:
→ Measurements in situ: non-invasive preparation of biological samples, without embedding and cutting, while probing the sample volume.
→ Measurement under cryo-conditions.
→ High brilliance nano-beams with high resolution (down to~10nm!)
→ Available X-ray techniques (2D mapping and 3D tomography):
• X-ray fluorescence: Mapping the (trace-) elemental distributions from sodium to uranium.
• X-ray absorption & phase contrast: imaging the topography of (usually transparent) biological objects.
• X-ray absorption spectroscopy: distinguishing the oxidation state of elements.
• X-ray diffraction: including crystallography.

The workshop will feature rapidly emerging fields in different branches of biology, medicine, physiology, bio-chemistry, etc. Leading scientists in the fields of cellular and molecular biology, medicine, ecology as well as X-ray microscopy, electron microscopy and imaging will address future developments in bio-imaging with synchrotron radiation. A strong emphasis will be placed on methods to analyse and image complex interactions in biological systems at molecular, cellular and organism levels. We are aiming to examine structures and dynamics of biological interactions, to reveal cellular functions, such as signalling cascades or metabolic pathways, and pathological changes on the cellular level. We look forward to discussing with you how your research could benefit from new instruments at PETRA III, a new high-brilliance synchrotron radiation source at the DESY site in Hamburg. You are very welcome to contribute with a short talk or poster, and especially with discussion. Please feel free to inform your colleagues who are interested in new bio-imaging methods.

Bio-imaging teams at PETRA III:

Tanja Dučić and Alke Meents (Beamline P11)
Gerald Falkenberg and Gerd Wellenreuther (Beamline P06)

    • 12:00 12:30
      Registration 30m
    • 12:30 12:40
      Opening remarks 10m
      Speaker: Edgar Weckert (DESY)
    • 12:40 12:50
      Short introduction 10m
      Speaker: Tanja DUCIC (DESY)
    • 12:50 13:20
      Correlated fluorescence and soft X-ray tomography: Imaging molecules with respect to cell structures 30m
      Speaker: Carolyn Larabell (University of California, San Francisco)
    • 13:20 13:50
      Chemical element imaging in cells: synchrotron radiation and particle induced X-ray emission 30m
      Speaker: Richard Ortega (CNRS and University of Bordeaux)
    • 13:50 14:10
      Beamline P11 - A Versatile Instrument for Imaging Biological Samples 20m
      Speaker: Alke Meents (DESY)
    • 14:10 14:30
      Coffee break 20m
    • 14:30 15:00
      Imaging of protein self-assembly 30m
      Speaker: Eckhard Mandelkow (Max-Planck-Unit for Structural Molecular Biology)
    • 15:00 15:20
      3-D Analysis of Microtubule Binding Factors in vitro and in situ 20m
      Speaker: Andreas Hoenger (University of Colorado, Boulder)
    • 15:20 15:40
      Green Light for Membrane Proteins 20m
      Speaker: Jan-Willem de Gier (University of Stockholm)
    • 15:40 16:00
      Metal-Peptide Conjugates for Cellular Uptake and Intra-cellular Localization 20m
      Speaker: Nils Metzler-Nolte (University of Bochum)
    • 16:00 16:20
      Coffee break 20m
    • 16:20 16:40
      Imaging targets in neurodegeneration and –regeneration 20m
      Speaker: Paul Lingor (University of Göttingen)
    • 16:40 17:00
      Spectroscopic features and their application in chemical state sensitive mapping of biological material 20m
      Speaker: Wolfram Meyer-Klaucke (EMBL)
    • 17:00 17:20
      Biochemical imaging of brain tumors using synchrotron radiation microprobe 20m
      Speaker: Magdalena Szczerbhowska-Boruchowska (AGH University of Science and Technology, Cracow)
    • 17:20 18:20
      Poster session 1h
    • 18:20 19:30
      Discussion 1h 10m
    • 19:30 20:00
      Departure to restaurant 30m
    • 20:00 22:00
      Dinner at "Werkstatt 3", Altona 2h
    • 09:00 09:20
      The Hard X-Ray Micro/Nano-Probe beamline P06 and its Application in Biology 20m
      Speaker: Gerald Falkenberg (DESY)
    • 09:20 09:40
      Quantitative imaging of metals in tissue and cells 20m
      Speaker: Wolf Osterode (Medical University of Vienna)
    • 09:40 10:00
      Synchrotron based techniques in the investigation of mechanisms underlying the neurodegenerative changes induced by pilocarpine evoked epileptic seizures and mechanical brain injury 20m
      Speaker: Joanna Chwiej (AGH University of Science and Technology, Cracow)
    • 10:00 10:20
      Manganese-enhanced magnetic resonance imaging: functional insights into the living mouse brain 20m
      Speaker: Susann Boretius (MPI for Biophysical Chemistry)
    • 10:20 10:40
      Infrared micro- and nano-spectroscopy of bio-materials and living cells 20m
      Speaker: Erik Bruendermann (University of Bochum)
    • 10:40 11:00
      Coffee break 20m
    • 11:00 11:20
      Actin-binding proteins of the malaria parasite - from structure to function 20m
      Speaker: Inari Kursula (CSSB)
    • 11:20 11:40
      New insights into the processes of virus/host interaction and virus assembly by the combination of systemic microscopy techniques with diffraction techniques and x-ray imaging? 20m
      Speaker: Heinrich Hohenberg (Heinrich-Pette-Institute)
    • 11:40 12:00
      Structural characterisation of the plant cell walls of various origin and their constituent polymers, using optical spectroscopy, scanning probe microscopy and X Ray Diffraction 20m
      Speaker: Ksenija Radotic (University of Belgrade)
    • 12:00 12:20
      An Integrative Approach to Study Structure-Function Relations in Biomedical Tissues 20m
      Speaker: Walther Schroeder (Forschungszentrum Jülich)
    • 12:20 12:40
      Hard X-ray Nanoprobe Investigations of the Metal Distribution within the Biological Model Organism Daphnia magna 20m
      Speaker: Björn de Samber (University of Ghent)
    • 12:40 13:40
      Lunch 1h
    • 13:45 14:30
      PETRA III tour 45m
    • 14:30 15:15
      Discussion 45m
    • 15:15 15:30
      Closing remarks 15m