Synchrotron Radiation for Bio-Imaging at PETRA III

29 Mar 2010, 12:00 → 30 Mar 2010, 15:30 Europe/Berlin

DESY, Notkestraße 85, 22607 Hamburg, Bldg. 28c, seminar room

Single isolated neural receptor cell in X-ray light: Distribution of K (red) and Cl (blue) [Dučić et al, 2009].

This meeting aims to bring scientists from biological and medical backgrounds together with synchrotron experts to establish a new interdisciplinary user community around the new high resolution beamlines P11 and P06 at PETRA III. Both beamlines are currently under construction and will enter the commissioning phase shortly after the workshop. The workshop is a unique opportunity for a hands-on introduction into the new possibilities for bio-imaging on the micro- and nano-scale at this facility. It will also be an excellent occasion to propose new experiments and collaborations, as well as to discuss future developments or requirements from the users. We are looking forward to addressing novel, groundbreaking biological and medical questions using synchrotron radiation.

We propose to offer opportunities such as:
→ Measurements in situ: non-invasive preparation of biological samples, without embedding and cutting, while probing the sample volume.
→ Measurement under cryo-conditions.
→ High brilliance nano-beams with high resolution (down to~10nm!)
→ Available X-ray techniques (2D mapping and 3D tomography):
• X-ray fluorescence: Mapping the (trace-) elemental distributions from sodium to uranium.
• X-ray absorption & phase contrast: imaging the topography of (usually transparent) biological objects.
• X-ray absorption spectroscopy: distinguishing the oxidation state of elements.
• X-ray diffraction: including crystallography.

The workshop will feature rapidly emerging fields in different branches of biology, medicine, physiology, bio-chemistry, etc. Leading scientists in the fields of cellular and molecular biology, medicine, ecology as well as X-ray microscopy, electron microscopy and imaging will address future developments in bio-imaging with synchrotron radiation. A strong emphasis will be placed on methods to analyse and image complex interactions in biological systems at molecular, cellular and organism levels. We are aiming to examine structures and dynamics of biological interactions, to reveal cellular functions, such as signalling cascades or metabolic pathways, and pathological changes on the cellular level. We look forward to discussing with you how your research could benefit from new instruments at PETRA III, a new high-brilliance synchrotron radiation source at the DESY site in Hamburg. You are very welcome to contribute with a short talk or poster, and especially with discussion. Please feel free to inform your colleagues who are interested in new bio-imaging methods.

Sincerely,
Bio-imaging teams at PETRA III:

Tanja Dučić and Alke Meents (Beamline P11)
Gerald Falkenberg and Gerd Wellenreuther (Beamline P06)

Monday 29 March

12:00 → 12:30 Registration

12:30 → 12:40 Opening remarks

Speaker: Edgar Weckert (DESY)

12:40 → 12:50 Short introduction

Speaker: Tanja DUCIC (DESY)

12:50 → 13:20 Correlated fluorescence and soft X-ray tomography: Imaging molecules with respect to cell structures

Speaker: Carolyn Larabell (University of California, San Francisco)

13:20 → 13:50 Chemical element imaging in cells: synchrotron radiation and particle induced X-ray emission

Speaker: Richard Ortega (CNRS and University of Bordeaux)

13:50 → 14:10 Beamline P11 - A Versatile Instrument for Imaging Biological Samples

Speaker: Alke Meents (DESY)

14:10 → 14:30 Coffee break

14:30 → 15:00 Imaging of protein self-assembly

Speaker: Eckhard Mandelkow (Max-Planck-Unit for Structural Molecular Biology)

15:00 → 15:20 3-D Analysis of Microtubule Binding Factors in vitro and in situ

Speaker: Andreas Hoenger (University of Colorado, Boulder)

15:20 → 15:40 Green Light for Membrane Proteins

Speaker: Jan-Willem de Gier (University of Stockholm)

15:40 → 16:00 Metal-Peptide Conjugates for Cellular Uptake and Intra-cellular Localization

Speaker: Nils Metzler-Nolte (University of Bochum)

16:00 → 16:20 Coffee break

16:20 → 16:40 Imaging targets in neurodegeneration and –regeneration

Speaker: Paul Lingor (University of Göttingen)

16:40 → 17:00 Spectroscopic features and their application in chemical state sensitive mapping of biological material

Speaker: Wolfram Meyer-Klaucke (EMBL)

17:00 → 17:20 Biochemical imaging of brain tumors using synchrotron radiation microprobe

Speaker: Magdalena Szczerbhowska-Boruchowska (AGH University of Science and Technology, Cracow)

17:20 → 18:20 Poster session

18:20 → 19:30 Discussion

19:30 → 20:00 Departure to restaurant

20:00 → 22:00 Dinner at "Werkstatt 3", Altona

Tuesday 30 March

09:00 → 09:20 The Hard X-Ray Micro/Nano-Probe beamline P06 and its Application in Biology

Speaker: Gerald Falkenberg (DESY)

09:20 → 09:40 Quantitative imaging of metals in tissue and cells

Speaker: Wolf Osterode (Medical University of Vienna)

09:40 → 10:00 Synchrotron based techniques in the investigation of mechanisms underlying the neurodegenerative changes induced by pilocarpine evoked epileptic seizures and mechanical brain injury

Speaker: Joanna Chwiej (AGH University of Science and Technology, Cracow)

10:00 → 10:20 Manganese-enhanced magnetic resonance imaging: functional insights into the living mouse brain

Speaker: Susann Boretius (MPI for Biophysical Chemistry)

10:20 → 10:40 Infrared micro- and nano-spectroscopy of bio-materials and living cells

Speaker: Erik Bruendermann (University of Bochum)

10:40 → 11:00 Coffee break

11:00 → 11:20 Actin-binding proteins of the malaria parasite - from structure to function

Speaker: Inari Kursula (CSSB)

11:20 → 11:40 New insights into the processes of virus/host interaction and virus assembly by the combination of systemic microscopy techniques with diffraction techniques and x-ray imaging?

Speaker: Heinrich Hohenberg (Heinrich-Pette-Institute)

11:40 → 12:00 Structural characterisation of the plant cell walls of various origin and their constituent polymers, using optical spectroscopy, scanning probe microscopy and X Ray Diffraction

Speaker: Ksenija Radotic (University of Belgrade)

12:00 → 12:20 An Integrative Approach to Study Structure-Function Relations in Biomedical Tissues

Speaker: Walther Schroeder (Forschungszentrum Jülich)

12:20 → 12:40 Hard X-ray Nanoprobe Investigations of the Metal Distribution within the Biological Model Organism Daphnia magna

Speaker: Björn de Samber (University of Ghent)

12:40 → 13:40 Lunch

13:45 → 14:30 PETRA III tour

14:30 → 15:15 Discussion

15:15 → 15:30 Closing remarks