Speaker
Prof.
Wolfgang J. Parak
(Universität Hamburg)
Description
In the context of biological environment nanoparticles are hybrid materials, which apart from the "bulk" particle also involve the corresponding surface chemistry and a corona of adsorbed molecules from the environment, such as proteins [1]. Upon cellular internalization the nanoparticles may degrade into their individual parts [2]. This process can be followed by using radiotracers [3], fluorescence [4], and mass spectroscopy [5]. Furthermore adsorption of proteins can be probed by fluorescence correlation spectroscopy [6] or nuclear magnetic resonance [7]. It will be discussed how such experiments could be also investigated using synchrotron radiation.
References
[1] S. Roy, Z. Liu, X. Sun, M. Gharib, H. Yan, Y. Huang, S. Megahed, M. Schnabel, D. Zhu, N. Feliu, I. Chakraborty, C. Sanchez-Cano, A. M. Alkilany, W. J. Parak, "Assembly and Degradation of Inorganic Nanoparticles in Biological Environments", Bioconjugate Chemistry 30, 2751−2762 (2019).
[2] N. Feliu, D. Docter, M. Heine, P. del Pino, S. Ashraf, J. Kolosnjaj-Tabi, P. Macchiarini, P. Nielsen, D. Alloyeau, F. Gazeau, R. H. Stauber, W. J. Parak, “In vivo degeneration and the fate of inorganic nanoparticles”, Chemical Society Reviews 45, 2440-2457 (2016).
[3] W. G. Kreyling, A. M. Abdelmonem, Z. Ali, F. Alves, M. Geiser, N. Haberl, R. Hartmann, S. Hirn, K. Kantner, D. Jimenez de Aberasturi, G. Khadem-Saba, J.-M. Montenegro, J. Rejman, T. Rojo, I. Ruiz de Larramendi, R. Ufartes, A. Wenk, W. J. Parak, "In vivo integrity of polymer-coated gold nanoparticles", Nature Nanotechnology 10, 619–623 (2015).
[4] C. Carrillo-Carrion, A. I. Bocanegra, B. Arnaiz, N. Feliu, D. Zhu, W. J. Parak, "Triple-Labeling of Polymer-Coated QuantumDots and Adsorbed Proteins for Tracing their Fate in Cell Cultures", ACS Nano 13, 4631-4639 (2019).
[5] Z. Liu, A. Escudero, C. Carrillo-Carrion, I. Chakraborty, D. Zhu, M. Gallego, W. J. Parak, N. Feliu, "Biodegradation of Bi-Labeled Polymer-Coated Rare-Earth Nanoparticles in Adherent Cell Cultures", Chemistry of Materials 32, 245-254 (2020).
[6] C. Röcker, M. Pötzl, F. Zhang, W. J. Parak, G. U. Nienhaus, "A quantitative fluorescence study of protein monolayer formation on colloidal nanoparticles", Nature Nanotechnology 4, 577-580 (2009).
[7] M. Carril, D. Padro, P. del Pino, C. Carrillo-Carrion, M. Gallego, W. J. Parak, "In situ detection of the protein corona in complex environments", Nature Communications 8, 1542 (2017).
Primary author
Prof.
Wolfgang J. Parak
(Universität Hamburg)
