Understanding intense XFEL interaction with matter is essential not only because of fundamental interest but also for interpreting experimental results. This presentation reviews recent experimental studies on intense x-ray interaction with matter using unique operation modes at SACLA (twin XFEL mode [1] and self-seeding mode [2]).
In particular, I will focus on XFEL-induced femtosecond...
The introduction of ultrafast and ultrabright X-ray free electron lasers (XFELs) has allowed us to observe the structural illumination of biological macromolecules unattainable in synchrotron radiation sources at an unprecedented temporal and spatial resolution. Here we report the first near-physiological temperature structure of a large ribosomal subunit (50S) isolated from Thermus...
Abstract
The iron-sulfur (Fe-S) cluster assembly is an essential mechanism for sufficing cellular needs for the Iron sulfur clusters, the ancient small protein cofactors, found in various organisms. The iron-sulfur cluster assembly machinary is known to be correlated with diverse cellular functions including electron transfer, gene expression, regulation, nitrogen fixation, RNA modification...
The concept of high-harmonic lasing at X-ray Free-Electron Laser (XFEL) facilities [1] opens new perspectives of generating ultrashort SASE pulses in ultrahard X-ray range up to 100 keV. The European XFEL (EuXFEL) facility is the worldwide unique hard XFEL based on superconducting accelerator operating at an electron energy of up to 17.5 GeV and producing electron bunches at MHz repetition...
Chronic myeloid leukemia (CML) is a kind of blood cancer and most CML patients have associated with a chromosomal anomaly with the BCR-ABL fusion oncogene, which occurs as a result of translocation between the Abelson murine leukemia (ABL1) gene on chromosome 9 and breakpoint cluster region (BCR) gene on chromosome 22. Imatinib mesylate is the first small molecule developed to target the...
We are investigating the formation of metallophilic bonds in luminescent stimuli-responsive compounds based on ligand-supported Au(I)/Ag(I)/Cu(I) complexes upon electronic transition to the emissive excited state. Combining pump-probe X-ray absorption spectroscopy with high energy resolution (HERFD-XAS) and wide-angle X-ray scattering (WAXS) in the solution we can visualize the local...
X-ray emission spectroscopy (XES) is a powerful tool for electronic structure characterization, it has been widely used in the study of 3d transition elements, while the K-edge XES spectra of 4d elements were rarely reported [1], even though they are greatly important for phase transition, photocatalyst, biology, geochemistry and etc. One of the main reasons for this is that the K emission...
Radiation damage is one of the major limitations of x-ray crystallography. XFELs provide ultrafast and ultrabright X-ray pulses allowing data collection without secondary radiation damage at ambient temperature. Here, we determined the crystal structure of Severe Acute Respiratory Syndrome CoronaVirus-2 main protease by serial femtosecond x-ray crystallography. To compare the structural...
Determining the high-resolution biomacromolecular structure is crucial for understanding protein function and dynamics. Serial crystallography is a new structural biology technique, but it is constrained fundamentally by the need for large samples or by the need for quick access to the scarce X-ray beamtime. The key challenge in serial crystallography continues to be obtaining a large number...
Determining the high-resolution biomacromolecular structure is crucial for understanding protein function and dynamics. Serial crystallography is a new structural biology technique, but it is constrained fundamentally by the need for large samples or by the need for quick access to the scarce X-ray beamtime. The key challenge in serial crystallography continues to be obtaining a large number...
Aminoglycosides are antibiotics that cause translational misreading of mRNA by binding to the A-site in the 30S ribosomal subunit. Together with the importance of antibiotics in the clinic, there are still missing details about their binding mode and structural dynamics on the decoding center of the 30S ribosomal subunit. Sisomicin is a precursor of structurally similar aminoglycoside...
Traf6 is a protein which affects NF-KB mediated transcription, hence it has a role in proliferation, differentiation, apoptosis and other cellular processes. Recent research has shown that, various forms of pancreatic cancer is related to Traf6 function, making the protein a good therapeutic target. A novel dithiol agent named SN1 shows good promise as a Traf6 inhibitor, as it can reduce the...
The Swedish Materials Science beamline (SMS) at PETRA III is dedicated to the exploitation of high energy synchrotron radiation (40-150 keV) for materials science applications, the key features of high energy X-rays being penetration power and compression of reciprocal space onto area detectors. The in-line branch (P21.2) has been designed for the investigation of both bulk materials and...
4$^{th}$ generation high-energy synchrotron photon sources offer unprecedented capabilities of probing matter during transient dynamics at high spatio-temporal scales using hard X-rays. The combination of high brilliance, short bunch duration (down to 60~ps) and high-energy of the extremely brilliant source at ESRF-EBS$^1$ opens the door of studying materials under extreme events of shock and...
The Structure of USP7 at Ambient Temperature by Using X-ray Crystallography
Nowadays cancer is a serious problem that doesn’t have very efficient treatment approaches. According to the world health organization (WHO) 10 million people died because of cancer in 2020. [1] Some mutations in DNA cause cancer and these cancer cells hide from cell cycle regulators and avoid cell death called...
We propose to develop, characterize and operate a superconducting undulator (SCU) afterburner consisting of 5 undulator modules (1 module = 2 times SCU coil of 2 m length and 1 phase shifter) at the SASE2 hard X-ray beamline of European XFEL. This afterburner has the potential to produce an output of more than 1010 ph/pulse at photon energies above 30 keV. The project is divided into the...
In recent years, molecular dynamics of macromolecules has benefitted immensely from the femtosecond timescales of X-ray Free Electron Lasers (XFELs)$\mathrm{^{[1]}}$. However, the progress for small unit cell systems has been slow. Thus, Dr. L. Krause and B. Svane have developed a data reduction pipeline for small unit cell systems, including peak hunting, indexing, integration, and...
The Pair Distribution Function (PDF) method interprets Total Scattering data in real space [1]. This distinguishes local structure from the long-range average using information contained within diffuse scattering. xFEL facilities provide the potential to apply this tool to pump-probe experiments and local structural dynamics on the native (sub-)picosecond response timescale of a material. ...
Time-resolved X-ray methods are widely used for monitoring time-resolved electronic and structural dynamics over the course of photochemical reactions. Since fundamental processes in physics, chemistry and biology occur in ultrashort time range (from 10e-12 to 10e-15 s), X-ray free electrons lasers (XFELs) development received much more attention in the last ten years. Several experiments have...
Ultrasonic liquid phase exfoliation has been identified as a promising processing route for manufacturing 2D functional materials in large scale. The ultrasonic cavitation bubble implosion plays a critical role in enabling 2D layer exfoliation. However, due to the highly transient implosion process occurring at µm length and sub-µs time scale, many fundamental issues in this process are either...
