15-18 March 2021
DESY
Europe/Berlin timezone

Low dose measurements for spatially resolved anomalous dispersion refinement

Not scheduled
20m
DESY

DESY

Poster contribution Bio-crystallography Bio-crystallography

Speaker

Dr Frank Lennartz (Helmholtz-Zentrum Berlin, Macromolecular Crystallography)

Description

Spatially resolved anomalous dispersion (SpReAD) refinement is a method to analyse the oxidation states of individual protein-bound metals and is based on collecting several x-ray diffraction datasets across an x-ray absorption edge. Such an approach can expose crystals to relatively high total dose of radiation. This can result in radiation damage, including reduction of metals.

Here, we have conducted experiments for SpReAD analysis on HZB MX beamline 14.1. To analyse the effect of dose on the result of the experiments, we have tested different transmission settings to vary the total dose per experiment and test whether data collected at low transmission are suitable for SpReAD refinement.

Primary author

Dr Frank Lennartz (Helmholtz-Zentrum Berlin, Macromolecular Crystallography)

Co-authors

Dr Jae-Hun Jeoung (Humboldt University of Berlin) Prof. Holger Dobbek (Humboldt University of Berlin) Dr Manfred Weiss (Helmholtz-Zentrum Berlin, Macromolecular Crystallography)

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